Difference between revisions of "Part:BBa K4341000:Design"

 
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===Design Notes===
 
===Design Notes===
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There were a lot of repeats in the sequence so ordering on IDT proved difficult
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rrnB P1 is an E.coli constitutive promoter that contains recognition sequences for holoenzymes containing sigma 70. This part was designed and chosen from literature in order to be used for high constitutive expression of a gene. ppGpp at basal levels has been shown to reduce strength of the rrnB P1 promoter.
  
  
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===Source===
 
===Source===
  
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E.coli
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<i>E. coli</i>
  
 
===References===
 
===References===
 +
Zhang X, Bremer H. Control of the Escherichia coli rrnB P1 promoter strength by ppGpp. J Biol Chem. 1995 May 12;270(19):11181-9. doi: 10.1074/jbc.270.19.11181. PMID: 7538113.

Latest revision as of 12:30, 12 October 2022


rrnB P1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

rrnB P1 is an E.coli constitutive promoter that contains recognition sequences for holoenzymes containing sigma 70. This part was designed and chosen from literature in order to be used for high constitutive expression of a gene. ppGpp at basal levels has been shown to reduce strength of the rrnB P1 promoter.


Source

E. coli

References

Zhang X, Bremer H. Control of the Escherichia coli rrnB P1 promoter strength by ppGpp. J Biol Chem. 1995 May 12;270(19):11181-9. doi: 10.1074/jbc.270.19.11181. PMID: 7538113.