Difference between revisions of "Part:BBa K4195180"

 
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__NOTOC__
 
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<partinfo>BBa_K4195180 short</partinfo>
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===Biology===
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This sequence is a conserved region of toxin gene ''pirB'' (''1''). It’s used as the detection target of RENDR system.<br/>
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'''Ribozyme ENabled Detection of RNA (RENDR)'''<br/>
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RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/>
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[[File:T--XMU-China--RENDR.png|400px]]<br/>
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'''Fig. 1 Schematic illustration of RENDR.'''<br/>
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===Usage and Design===
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This part is used as the target of the detection system <partinfo>BBa_K4195187</partinfo>、<partinfo>BBa_K4195188</partinfo>、<partinfo>BBa_K4195189</partinfo> and <partinfo>BBa_K4195190</partinfo>. We build the circuit similar as <partinfo>BBa_K4195178</partinfo>. <br/>
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====Reference====
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1. J. M. S. Lazarte ''et al.'' Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus vannamei'' Shrimp. ''Vaccines (Basel)''. '''9''',  (2021).
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4195180 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4195180 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K4195180 parameters</partinfo>
 
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Latest revision as of 15:19, 12 October 2022

Biology

This sequence is a conserved region of toxin gene pirB (1). It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
T--XMU-China--RENDR.png
Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the detection system BBa_K4195187、BBa_K4195188、BBa_K4195189 and BBa_K4195190. We build the circuit similar as BBa_K4195178.

Reference

1. J. M. S. Lazarte et al. Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel). 9, (2021).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 207
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]