Difference between revisions of "Part:BBa K4115021:Design"
 
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===Design Notes===
 
===Design Notes===
−
Using sRNA is a convenient and efficient way to silence a gene, that why we first come up with this idea to increase sucrose in Synechococcus.
+
Using sRNA is a convenient and efficient way to silence a gene, that why we first come up with this idea to increase sucrose in <i>S.elongatus</i> HL7942.
  
  
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===Source===
 
===Source===
  
−
The basic design of the composite part is inspired by the article 'Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria'.The glgA sRNA(BBa_K4115015) is designed by our group. The sequence of fragments is partially synthesized by the company. The whole composite part is stitched with fragments and the backbone of PUC57.
+
The basic design of the composite part is inspired by the article 'Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria' [1]. The glgA sRNA [https://parts.igem.org/Part:BBa_K4115015 (BBa_K4115015)] is designed by our group. The sequence of fragments is partially synthesized by the company. The whole composite part is stitched with fragments and the backbone of PUC57.
  
 
===References===
 
===References===
 +
[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16

Latest revision as of 08:42, 4 October 2022


glgA gene silence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
    Illegal XbaI site found at 1903
    Illegal PstI site found at 6912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
    Illegal PstI site found at 6912
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
    Illegal XbaI site found at 1903
    Illegal PstI site found at 6912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
    Illegal XbaI site found at 1903
    Illegal PstI site found at 6912
    Illegal AgeI site found at 2068
    Illegal AgeI site found at 6284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2065
    Illegal BsaI.rc site found at 6281


Design Notes

Using sRNA is a convenient and efficient way to silence a gene, that why we first come up with this idea to increase sucrose in S.elongatus HL7942.


Source

The basic design of the composite part is inspired by the article 'Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria' [1]. The glgA sRNA (BBa_K4115015) is designed by our group. The sequence of fragments is partially synthesized by the company. The whole composite part is stitched with fragments and the backbone of PUC57.

References

[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16