Difference between revisions of "Part:BBa K4195070"

Latest revision as of 09:04, 13 October 2022

pirB_i

This sequence is a conserved region of toxin gene pirB(1). It’s used as the detection target of RENDR system.

Biology

Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform(1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.

Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the RENDR detection system. For toxin pirB, we designed BBa_K4195143, BBa_K4195144, BBa_K4195147, BBa_K4195148, BBa_K4195158, BBa_K4195159, BBa_K4195162, BBa_K4195163, BBa_K4195170, BBa_K4195171, BBa_K4195174, BBa_K4195175. Other related parts are as following: BBa_K4195149, BBa_K4195150, BBa_K4195164, BBa_K4195165, BBa_K4195166, BBa_K4195187, BBa_K4195188, BBa_K4195189, BBa_K4195190.

Reference

1. J. M. S. Lazarte et al., Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel) 9, (2021).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4195070 short</partinfo>
-none
+This sequence is a conserved region of toxin gene <i>pirB</i>(<i>1</i>). It’s used as the detection target of RENDR system.
+===Biology===
-<!-- Add more about the biology of this part here
+<b>Ribozyme ENabled Detection of RNA (RENDR)</b>
-===Usage and Biology===
+<br>
+RENDR is a high-performing, plug-and-play RNA-sensing platform(<i>1</i>). RENDR utilizes a split variant of the <i>Tetrahymena thermophila</i> ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
+<br>
+[[File:T--XMU-China--RENDR.png|400px]]
+<br>
+<b>Fig. 1 Schematic illustration of RENDR.</b>
+===Usage and Design===
+<br>
+This part is used as the target of the RENDR detection system. For toxin <i>pirB</i>, we designed <partinfo>BBa_K4195143</partinfo>, <partinfo>BBa_K4195144</partinfo>, <partinfo>BBa_K4195147</partinfo>, <partinfo>BBa_K4195148</partinfo>, <partinfo>BBa_K4195158</partinfo>, <partinfo>BBa_K4195159</partinfo>, <partinfo>BBa_K4195162</partinfo>, <partinfo>BBa_K4195163</partinfo>, <partinfo>BBa_K4195170</partinfo>, <partinfo>BBa_K4195171</partinfo>, <partinfo>BBa_K4195174</partinfo>, <partinfo>BBa_K4195175</partinfo>. Other related parts are as following: <partinfo>BBa_K4195149</partinfo>, <partinfo>BBa_K4195150</partinfo>, <partinfo>BBa_K4195164</partinfo>, <partinfo>BBa_K4195165</partinfo>, <partinfo>BBa_K4195166</partinfo>, <partinfo>BBa_K4195187</partinfo>, <partinfo>BBa_K4195188</partinfo>, <partinfo>BBa_K4195189</partinfo>, <partinfo>BBa_K4195190</partinfo>.
+===Reference===
+<br/>
+. J. M. S. Lazarte <i>et al.</i>, Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> Shrimp. <i>Vaccines (Basel)</i> <b>9</b>,  (2021).
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