Difference between revisions of "Part:BBa K176039:Design"
(New page: __NOTOC__ <partinfo>BBa_K176039 short</partinfo> <partinfo>BBa_K176039 SequenceAndFeatures</partinfo> ===Design Notes=== Designed with our software tool, which is modified from primer3 ...) |
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| − | Reverse complement of VR5 ( | + | Reverse complement of VR5 (K176049). Picked from random sequences. |
===References=== | ===References=== | ||
Latest revision as of 07:56, 13 August 2009
Reverse primer VR5 (K176049) binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
Main parameters for picking VR3~VR12:
Size: 20 bp (N19 + G or C)
Tm: VF2 Tm ±1℃
GC%: 50%
Max Self Complementarity: 3 bp
Max 3' Self Complementarity: 2 bp
Max Pair Complementarity: 3 bp
Max 3' Pair Complementarity: 2 bp
Max 3' Stability: 3.36 kcal/mol
Max Template(pSB1A3) Mispriming: 6 bp
Max Mispriming With Other VR3~VR12 Sites: 4 bp
Source
Reverse complement of VR5 (K176049). Picked from random sequences.