Difference between revisions of "Part:BBa K4195018"

 
 
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<partinfo>BBa_K4195018 short</partinfo>
 
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<i>AraC</i> is an operon of arabinose promoter (<partinfo>BBa_I0500</partinfo>).
  
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===Biology===
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===Usage and Biology===
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In the absence of arabinose, the AraC dimer adopts a different conformation and binds to <i>araO<sub>2</sub></i> and <i>araI<sub>1</sub></i>. In this position, there is no monomer at site <i>araI<sub>2</sub></i>, and so the protein cannot activate the <i>araBAD</i> promoter (<i>araP</i><sub>BAD</sub>). When arabinose is present, arabinose binds to AraC, changing the shape of that activator so that it binds as a dimer to <i>araI<sub>1</sub></i> and <i>araI<sub>2</sub></i>. This places one monomer of AraC close to the promoter from which it can activate transcription.
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===Usage===
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We planned to add another araC gene after <partinfo>BBa_I0500</partinfo> in order to achieve the stricter regulation of this inducible promoter.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4195018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4195018 SequenceAndFeatures</partinfo>

Latest revision as of 12:10, 12 October 2022


araC

AraC is an operon of arabinose promoter (BBa_I0500).

Biology

In the absence of arabinose, the AraC dimer adopts a different conformation and binds to araO2 and araI1. In this position, there is no monomer at site araI2, and so the protein cannot activate the araBAD promoter (araPBAD). When arabinose is present, arabinose binds to AraC, changing the shape of that activator so that it binds as a dimer to araI1 and araI2. This places one monomer of AraC close to the promoter from which it can activate transcription.

Usage

We planned to add another araC gene after BBa_I0500 in order to achieve the stricter regulation of this inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]