Difference between revisions of "Part:BBa K4259000:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
two overhang sequences with the restriction enzymes BamHI and NdeI were designed and added to the sequence
+
Codon optimization was performed for the expression of the gene in E. coli.
  
  
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===Source===
 
===Source===
  
−
aattagttaactacgcatctggctgctcttgtggtgctgactgcaaatgtgcttccgaaactgaatgcaaat
+
The sequence of Cup1 is from the UniProt database.
  
 
===References===
 
===References===

Latest revision as of 07:33, 27 September 2022


Cup1 for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed for the expression of the gene in E. coli.


Source

The sequence of Cup1 is from the UniProt database.

References