Difference between revisions of "Part:BBa K4220000"

Latest revision as of 13:07, 26 September 2022

FZD5 sp-V5-RTAC

RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.

ZNRF3+linker+LGR4+human IgGH3 Fc

1.Better interaction with 4 RSPOs--changing the length of linker:

2.Better secretion capability--changing the type of signaling peptide

3.Better stability--adding an IGg Fc tag

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 70
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1254
Illegal BamHI site found at 2171
Illegal XhoI site found at 2162
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2274
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 124
Illegal BsaI site found at 1451
Illegal BsaI.rc site found at 1139

@@ Line 9: / Line 9: @@
 ZNRF3+linker+LGR4+human IgGH3 Fc
 ==The design and improvement of RTAC:==
-: Better interaction with 4 RSPOs--changing the length of linker
+.Better interaction with 4 RSPOs--changing the length of linker:
-a
-: Better secretion capability--changing the type of signaling peptide
-: Better stability--adding an IGg Fc tag
+[[File:RTAC1.jpg|550px|options|caption]]
+.Better secretion capability--changing the type of signaling peptide
+[[File:rtac2.jpg|550px|options|caption]]
+.Better stability--adding an IGg Fc tag
+[[File:rtac3.jpg|550px|options|caption]]
 <!-- Add more about the biology of this part here