Difference between revisions of "Part:BBa K4202007:Experience"
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we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. | we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. | ||
| − | <div align="center">[[File:GLL-5.png]]</div> | + | From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor. |
| − | See the [https://2022.igem.wiki/zju-china/results] 2022 wiki for more details. | + | <div align="center">[[File:GLL-5.png|600px]]</div> |
| + | <p><div align="center"><b>Fig 1-1</b>Characterization of Oxygen-limitation induced promotor by SDS-PAGE</div></p> | ||
| + | |||
| + | See the [https://2022.igem.wiki/zju-china/results] ZJU-China 2022 wiki for more details. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:17, 26 September 2022
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K4202007
we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor.
See the [1] ZJU-China 2022 wiki for more details.
User Reviews
UNIQ523ebef314008eaf-partinfo-00000000-QINU UNIQ523ebef314008eaf-partinfo-00000001-QINU