Difference between revisions of "Part:BBa K4348057:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Contains an EcoRI site, so standard biobrick assembly is not possible
+
Contains an EcoRI site, so standard biobrick assembly is not possible. Golden gate or gibson assembly are recommended, or the use of other restriction enzymes.
  
 
===Source===
 
===Source===

Latest revision as of 17:20, 25 September 2022


SUMO-his


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 281
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 281
    Illegal BglII site found at 170
    Illegal BamHI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains an EcoRI site, so standard biobrick assembly is not possible. Golden gate or gibson assembly are recommended, or the use of other restriction enzymes.

Source

Saccharomyces cerevisiae

References