Difference between revisions of "Part:BBa K3743019"

Latest revision as of 09:52, 29 September 2022

gRNA Binding site

Part Description

Complementary part to crRNA present down stream to the target gene

Usage

we employed this part to attract our regulatory system Cas12g guided by specific RNA to PAH mRNA that we want to stop its translation in case of overexpression of PAH or high levels OF tyrosine.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K4140014 short</partinfo>
+<partinfo>BBa_K3743019 short</partinfo>
-Variable part of the guide RNA to the target sequence or gene
+==Part Description==
+Complementary part to crRNA present down stream to the target gene
+==Usage==
+we employed this part to attract our regulatory system Cas12g guided by specific RNA  to PAH mRNA that we want to stop its translation in case of overexpression of PAH  or high levels OF tyrosine.
+==Literature Characterization==
+[[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]]
+<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+==References==
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
@@ Line 10: / Line 21: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K4140014 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K3743019 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K4140014 parameters</partinfo>
+<partinfo>BBa_K3743019 parameters</partinfo>
 <!-- -->