Difference between revisions of "Part:BBa K3743020"

 
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Constant part of the guide RNA necessary for cas12g binding
 
  
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==Part Description==
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A guide RNA (gRNA) is an RNA that is responsible for acting as a guide for enzymes that target DNA or RNA. Guide RNA targets their sequences by simple complementary base pairing of the target specific regions and it is used more specifically in applications that require targeted editing of DNA such as found and present in the CRISPR-Cas9 or Cas12 system.
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<br>
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==Usage==
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It is the RNA domain of the CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle we use this part to guide Cas12g protein to the complimentary site on mRNA of PAH to inhibit it’s expression when phenylalanine level is normal or to prevent over expression of PAH.
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==Literature Characterization==
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[[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]]
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==References==
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3743020 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4140013 parameters</partinfo>
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<partinfo>BBa_K3743020 parameters</partinfo>
 
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Latest revision as of 09:42, 29 September 2022


gRNA


Part Description

A guide RNA (gRNA) is an RNA that is responsible for acting as a guide for enzymes that target DNA or RNA. Guide RNA targets their sequences by simple complementary base pairing of the target specific regions and it is used more specifically in applications that require targeted editing of DNA such as found and present in the CRISPR-Cas9 or Cas12 system.

Usage

It is the RNA domain of the CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle we use this part to guide Cas12g protein to the complimentary site on mRNA of PAH to inhibit it’s expression when phenylalanine level is normal or to prevent over expression of PAH.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]