Difference between revisions of "Part:BBa K176096:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176096 short</partinfo>
 
<partinfo>BBa_K176096 short</partinfo>
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pCon 0.70(J23101) generates LuxR.
 
pCon 0.70(J23101) generates LuxR.
  
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pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
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pLux/Tet(K176000) generates lacZalpha-ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
  
  

Latest revision as of 05:06, 8 October 2009

Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 856
    Illegal NheI site found at 879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2323
    Illegal BsaI.rc site found at 1834


Design Notes

Input: AHL

Output1: AHL

Output2: Cell Density

Design:

pCon 0.15(K176008) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates lacZalpha-ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.


Source

K176085 K176063

References