Difference between revisions of "Part:BBa K4134002"

 
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<partinfo>BBa_K4134002 short</partinfo>
 
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==Description==
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[[Image:Nanjing-China-AgBP-affinity-list.jpeg|400px|thumb|Ag(I) affinity values of the biomolecules]]
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AgBP2 is a typical silver-binding peptide consisting of 12 amino acids(EQLGVRKELRGV).</p>
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<p>
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The silver-binding protein AgBP2 was selected from a combinatorial peptide display library and it presents a higher Ag(I) affinity value (Ka= 6.30 ±0.5 x10^7) than many other typical silver-binding proteins, such as Ag4 and AgP35. </p>
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AgBP2 captures silver ions and paves the way for a new approach to synthesizing silver nanoparticles(AgNPs).And in our project, AgNPs accelerate extracellular electron transfer.</p>
  
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We read about the silver-binding peptide AgBP2 selected from a combinatorial peptide display library and we engineered it onto the Shewanella membrane protein (bpfA-AgBP2). When localized to the periplasm of a laboratory strain of Shewanella, it confers tolerance to silver nitrate and results in the decoration of cells with electron-dense nanoparticles.
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AgBP2 captures silver ions and paves the way for a new approach to synthesizing silver nanoparticles which accelerates extracellular electron transfer.
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==Reference==
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<p>
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[1] Li, Xin-Yi, Xiao-Dong Zhou, and Ji-Ming Hu. "A straightforward and reliable evaluation of Ag (I) binding affinity mediated by a peptide ligand for constructing an efficient sensing platform." Talanta 226 (2021): 122089.</p>
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<p>
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[2] Hall Sedlak, Ruth, et al. "Engineered Escherichia coli silver-binding periplasmic protein that promotes silver tolerance." Applied and environmental microbiology 78.7 (2012): 2289-2296.</p>
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4134002 SequenceAndFeatures</partinfo>
 
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Latest revision as of 06:26, 12 October 2022


AgBP2

Description

Ag(I) affinity values of the biomolecules

AgBP2 is a typical silver-binding peptide consisting of 12 amino acids(EQLGVRKELRGV).

The silver-binding protein AgBP2 was selected from a combinatorial peptide display library and it presents a higher Ag(I) affinity value (Ka= 6.30 ±0.5 x10^7) than many other typical silver-binding proteins, such as Ag4 and AgP35.

AgBP2 captures silver ions and paves the way for a new approach to synthesizing silver nanoparticles(AgNPs).And in our project, AgNPs accelerate extracellular electron transfer.

Reference

[1] Li, Xin-Yi, Xiao-Dong Zhou, and Ji-Ming Hu. "A straightforward and reliable evaluation of Ag (I) binding affinity mediated by a peptide ligand for constructing an efficient sensing platform." Talanta 226 (2021): 122089.

[2] Hall Sedlak, Ruth, et al. "Engineered Escherichia coli silver-binding periplasmic protein that promotes silver tolerance." Applied and environmental microbiology 78.7 (2012): 2289-2296.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]