Difference between revisions of "Part:BBa K4235016"
 
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[[Image:Vector_sbu.jpg|700px|center|]]
 
[[Image:Vector_sbu.jpg|700px|center|]]
  
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The Human Protein S gene PROS1 () was inserted into this LIC cloning vector and was transformed into '''E.coli''' strains Bl21 and origami B for expression.  
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The Human Protein S gene PROS1 <partinfo>BBa_K2927011</partinfo> was inserted into this LIC cloning vector and was transformed into E.coli strains Bl21 and origami B for expression.  
  
 
This vector contains the following parts: <br>
 
This vector contains the following parts: <br>
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*T7 Promoter: <partinfo>BBa_K4235012</partinfo> <br>
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*T7 Promoter: <partinfo>BBa_J64997</partinfo> <br>
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*RBS: <partinfo>BBa_K4235014</partinfo> <br>
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*RBS: <partinfo>BBa_K2927001</partinfo> <br>
 
*Ampicillin resistance cassette (AmpR promoter+CDS): <partinfo>BBa_K4235029</partinfo> <br>
 
*Ampicillin resistance cassette (AmpR promoter+CDS): <partinfo>BBa_K4235029</partinfo> <br>
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*T7 terminator: <partinfo>BBa_K4235013</partinfo>
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*T7 terminator: <partinfo>BBa_B0016</partinfo>
  
  

Latest revision as of 14:56, 27 September 2022


pET His6 LIC cloning vector (2Bc-T)

Vector sbu.jpg

The Human Protein S gene PROS1 BBa_K2927011 was inserted into this LIC cloning vector and was transformed into E.coli strains Bl21 and origami B for expression.

This vector contains the following parts:


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4105
    Illegal PstI site found at 750
    Illegal PstI site found at 4090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4543
    Illegal PstI site found at 750
    Illegal PstI site found at 4090
    Illegal NotI site found at 4081
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3990
    Illegal BamHI site found at 4207
    Illegal XhoI site found at 4098
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4105
    Illegal PstI site found at 750
    Illegal PstI site found at 4090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4105
    Illegal PstI site found at 750
    Illegal PstI site found at 4090
    Illegal NgoMIV site found at 3076
    Illegal NgoMIV site found at 3430
    Illegal NgoMIV site found at 3590
    Illegal NgoMIV site found at 3958
    Illegal NgoMIV site found at 4054
    Illegal NgoMIV site found at 4237
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 924
    Illegal BsaI.rc site found at 4029
    Illegal SapI.rc site found at 2006