Difference between revisions of "Part:BBa K4115009:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NS3-2 was used together with NS3-1 to construct plasmids required for Synechococcus.
 
  
 +
NS3-2 and NSI-3-1 are a pair of commonly used homology arms in <i>S.elongatus</i>.
  
  
 
===Source===
 
===Source===
  
−
The sequence of NS3-2 is gained from the genome of Synechococcus 2973 and the fragment we use is artificially synthesized by the company.
+
The sequence of NS3-2 is gained from a NS3-targeting plasmid [1] and the fragment we use is artificially synthesized by the company.
  
 
===References===
 
===References===
 +
[1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321.

Latest revision as of 02:39, 4 October 2022


NS3-2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NS3-2 and NSI-3-1 are a pair of commonly used homology arms in S.elongatus.


Source

The sequence of NS3-2 is gained from a NS3-targeting plasmid [1] and the fragment we use is artificially synthesized by the company.

References

[1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321.