Difference between revisions of "Part:BBa K4115010:Design"
(→Source) |
(→Design Notes) |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| − | |||
| + | NSI-US and NSI-DS are a pair of commonly used homology arms in <i>S.elongatus</i>. | ||
===Source=== | ===Source=== | ||
| − | The sequence is gained from the genome of Synechococcus | + | The sequence is gained from the genome of <i>Synechococcus</i> 2973 [1] and the fragment we use is synthesized by the company. |
===References=== | ===References=== | ||
| + | [1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16 | ||
Latest revision as of 02:26, 4 October 2022
NSI-US
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 333
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 520
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 333
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 333
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
NSI-US and NSI-DS are a pair of commonly used homology arms in S.elongatus.
Source
The sequence is gained from the genome of Synechococcus 2973 [1] and the fragment we use is synthesized by the company.
References
[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16