Difference between revisions of "Part:BBa K4399000:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | We employed PAtUBI5 to drive expression of GFP, as a reporter, which confers constitutive expression of GFP. So successful transformants can be screened using GFP fluorescence. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | From Arabidopsis (Arabidopsis thaliana) genomic sequence. | |
| + | |||
===References=== | ===References=== | ||
Latest revision as of 01:49, 28 September 2022
PAtUBI5
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1342
Illegal XbaI site found at 696
Illegal XbaI site found at 1579 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1342
Illegal NheI site found at 1244 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1342
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1342
Illegal XbaI site found at 696
Illegal XbaI site found at 1579 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1342
Illegal XbaI site found at 696
Illegal XbaI site found at 1579 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We employed PAtUBI5 to drive expression of GFP, as a reporter, which confers constitutive expression of GFP. So successful transformants can be screened using GFP fluorescence.
Source
From Arabidopsis (Arabidopsis thaliana) genomic sequence.