Difference between revisions of "Part:BBa K4461000:Design"
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The sequence we cloned has to include all transcriptional factors for the promoter. | The sequence we cloned has to include all transcriptional factors for the promoter. | ||
Also, we used BBa_B0034 as the RBS, so we didn't clone the RBS from the genomic DNA. | Also, we used BBa_B0034 as the RBS, so we didn't clone the RBS from the genomic DNA. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 12:42, 11 October 2022
dusB-fis promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We cloned a larger fragment that included the promoter, then used another pair of primers to clone the promoter specifically. Therefore, we can avoid the length of the unintended bands being too similar to distinguish easily. The sequence we cloned has to include all transcriptional factors for the promoter. Also, we used BBa_B0034 as the RBS, so we didn't clone the RBS from the genomic DNA.
Source
The dusB-fis promoter is from E.coli-K12 MG1655 genomic DNA.