Difference between revisions of "Part:BBa K4461000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We cloned a larger fragment which include the promoter, then use another pair of primer to specify clone the promoter. Therefore we can avoid the length of the unintended bands are  too similar to distinct easily.    The sequence we cloned have to include all transcriptional factors for the promoter.    We used BBa_B0034 as the RBS of the promoter, so we didn't cloned the RBS from the genomic DNA.
+
We cloned a larger fragment that included the promoter, then used another pair of primers to clone the promoter specifically.
−
 
+
Therefore, we can avoid the length of the unintended bands being too similar to distinguish easily.     
−
 
+
The sequence we cloned has to include all transcriptional factors for the promoter.     
 +
Also, we used BBa_B0034 as the RBS, so we didn't clone the RBS from the genomic DNA.
  
  

Latest revision as of 12:42, 11 October 2022


dusB-fis promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned a larger fragment that included the promoter, then used another pair of primers to clone the promoter specifically. Therefore, we can avoid the length of the unintended bands being too similar to distinguish easily. The sequence we cloned has to include all transcriptional factors for the promoter. Also, we used BBa_B0034 as the RBS, so we didn't clone the RBS from the genomic DNA.


Source

The dusB-fis promoter is from E.coli-K12 MG1655 genomic DNA.

References