Difference between revisions of "Part:BBa K4159002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Description: This is a common consensus promoter that can be induced by IPTG. The following sequence after the promoter sequence is derived from the plasmid sequence used in the Chen et al. (2021) paper. | ||
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| + | The part has been used for ribosome display as well as the promoter for GFP binding DARPin expression. Check out the parts BBa_K4159012 and BBa_K4159000 for a more detailed description. | ||
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Latest revision as of 16:25, 1 October 2022
T7 promoter
Promoter for bacteriophage T7 RNA polymerase. With added stem-loop containing XbaI cutting site.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 21
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 21
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 21
- 1000COMPATIBLE WITH RFC[1000]