Difference between revisions of "Part:BBa K4235008"
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| − | This | + | This is the coding sequence of the Ampicillin Resistance gene which is used within the plasmid <partinfo>BBa_K4235002</partinfo> and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter (add link). This marker was used for the selection of successful transformants after cloning our gene insert (add link) in the plasmid pFastBac. |
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Latest revision as of 02:06, 13 September 2022
Ampicillin Resistance Gene
Introduction
Ampicillin is one of the most common selection markers used in synthetic biology. Its structure contains a β-lactam ring that inhibits proteins involved in bacterial peptidoglycan synthesis. As a result, bacteria cannot synthesize new peptidoglycan which disrupts the formation of cell walls and leads to cell death. The ampicillin resistance gene encodes for the enzyme β-lactamase, which cleaves the β-lactam ring and inactivates the ampicillin, thereby providing resistance to the bacteria.
Usage and Biology
This is the coding sequence of the Ampicillin Resistance gene which is used within the plasmid BBa_K4235002 and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter (add link). This marker was used for the selection of successful transformants after cloning our gene insert (add link) in the plasmid pFastBac.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 716