Difference between revisions of "Part:BBa K4370004"
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The PRK (phosphoribulokinase or ribulose-5-phosphate kinase) is a phosphotransferase that catalyze the synthesis of RuBP (D-ribulose-1,5-bisphosphate) using ATP and D-ribulose-5-phosphate as a substrate. | The PRK (phosphoribulokinase or ribulose-5-phosphate kinase) is a phosphotransferase that catalyze the synthesis of RuBP (D-ribulose-1,5-bisphosphate) using ATP and D-ribulose-5-phosphate as a substrate. | ||
− | This enzyme closes the Calvin cycle by regenerating RuBP, the substrate of the RuBisCO. Its activity plays a key role in directing glucose to the RuBisCO-based pathway. PRK expression without RuBisCO may be toxic to cells. This observation is the basis for phenotypic assays to characterize the activity of PRK and/or RuBisCO (when both are introduced into cells | + | This enzyme closes the Calvin cycle by regenerating RuBP, the substrate of the RuBisCO. Its activity plays a key role in directing glucose to the RuBisCO-based pathway. PRK expression without RuBisCO may be toxic to cells. This observation is the basis for phenotypic assays to characterize the activity of PRK and/or RuBisCO (when both are introduced into cells) (Parikh <i>et al., Protein Eng Des Sel</i>, 2007). |
[[Image:T--Go-Paris-Saclay-BBa_K4370004-2.png|500px|thumb|left|'''Figure :''' Reaction catalyzed by the PRK]] | [[Image:T--Go-Paris-Saclay-BBa_K4370004-2.png|500px|thumb|left|'''Figure :''' Reaction catalyzed by the PRK]] | ||
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Latest revision as of 07:59, 11 September 2022
PRK-STRBO
This parts encodes the phosphoribulokinase (PRK) of Streptomyces bottropensis ATCC 25435. The co-expression of this part with the form I RuBisCO large (BBa_K4370000, BBa_K4370002) and small (BBa_K4370001, BBa_K4370003) subunits of the RuBisCO of this strain may help to implement the Calvin cycle in bacterial chassis of interest such as E. coli .
Usage and Biology
The PRK (phosphoribulokinase or ribulose-5-phosphate kinase) is a phosphotransferase that catalyze the synthesis of RuBP (D-ribulose-1,5-bisphosphate) using ATP and D-ribulose-5-phosphate as a substrate.
This enzyme closes the Calvin cycle by regenerating RuBP, the substrate of the RuBisCO. Its activity plays a key role in directing glucose to the RuBisCO-based pathway. PRK expression without RuBisCO may be toxic to cells. This observation is the basis for phenotypic assays to characterize the activity of PRK and/or RuBisCO (when both are introduced into cells) (Parikh et al., Protein Eng Des Sel, 2007).
References
Monal R. Parikh, Dina N. Greene, Kristen K. Woods, and Ichiro Matsumura, “Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli”, Protein Engineering, Design and Selection 2006 Mar; 19(3): 113–119
https://doi-org.insb.bib.cnrs.fr/10.1093/protein/gzj010
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]