Difference between revisions of "Part:BBa K4501005"

Latest revision as of 10:11, 12 October 2022

MSCV 5' module

This 5' module codifies for the main 5' upstream components needed in a lentiviral transfer plasmid. Spceifically, it encodes for the T3 and RSV promoters, 5' LTR, psi sequence, RRE and gp41 peptide. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 780

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4501005 short</partinfo>
-Encodes for the 5' LTR and the psi sequences, used on the 5' terminus of lentiviral transfer plasmids. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.
+This 5' module codifies for the main 5' upstream components needed in a lentiviral transfer plasmid. Spceifically, it encodes for the T3 and RSV promoters, 5' LTR, psi sequence, RRE and gp41 peptide. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part can be used as the 5' module to be assembled into the pJUMP backbones and transform them into a lentiviral transfer plasmid, together with the 3' module (BBa_K4501006)
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