Difference between revisions of "Part:BBa K4362064"

Latest revision as of 15:58, 10 October 2022

Synthetic terminator - 1

The following three elements were previously shown to be required for 3'-end formation of mRNA in the Saccharomyces cerevisiae: 3’-end formation of yeast mRNAs involves signals having the following three degenerate elements: (i) the efficiency element, which functions by enhancing the efficiency of positioning elements; (ii) the positioning element, which positions the poly(A) site; and (iii) the actual site of polyadenylation.[1]

The sequences between these sequences can be shortened by us, and reducing the homology of these sequences can reduce the repeatability. The filling of sequences is random, based on the research of Murray MacPherson et al [2].

Reference [1]. Guo, Z. J., and Sherman, F. (1996) Signals sufficient for 3′-end formation of yeast mRNA. Mol. Cell. Biol. 16, 2772−2776. [2]. MacPherson, M.; Saka, Y. Short Synthetic Terminators for Assembly of Transcription Units in Vitro and Stable Chromosomal Integration in Yeast S. cerevisiae. ACS Synth Biol 2017, 6 (1), 130-138.

Secondary Structure

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

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 <partinfo>BBa_K4362064 short</partinfo>
-* This is a terminator used to end the transcription of the sequence of tRNA. In the project of Tianjin 2022, it is used to terminate the transcription of tRNA.
+* The following three elements were previously shown to be required for 3'-end formation of mRNA in the Saccharomyces cerevisiae: 3’-end formation of yeast mRNAs involves signals having the following three degenerate elements: (i) the efficiency element, which functions by enhancing the efficiency of positioning elements; (ii) the positioning element, which positions the poly(A) site; and (iii) the actual site of polyadenylation.[1]
+The sequences between these sequences can be shortened by us, and reducing the homology of these sequences can reduce the repeatability. The filling of sequences is random, based on the research of Murray MacPherson et al [2].
+Reference
+[1].	Guo, Z. J., and Sherman, F. (1996) Signals sufficient for 3′-end formation of yeast mRNA. Mol. Cell. Biol. 16, 2772−2776.
+[2].	MacPherson, M.; Saka, Y. Short Synthetic Terminators for Assembly of Transcription Units in Vitro and Stable Chromosomal Integration in Yeast S. cerevisiae. ACS Synth Biol 2017, 6 (1), 130-138.
 |}