Difference between revisions of "Part:BBa K4361114"

 
 
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<partinfo>BBa_K4361114 short</partinfo>
 
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This part shows the <i>blc</i> operator sequence  after site-directed mutagenesis of the ([[Part:BBa_K4361111]]) with primers [[Part:BBa_K4361112]] and [[Part:BBa_K4361113]], resulting in the deletion of the adenine in position 20.
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It is known from literature that this mutation disrupts the binding between BlcR and the <i> blc </i> operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.
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The mutated <i> blc </i> operator sequence is incorperated in a superfolded GFP production plasmid, see [[Part:BBa_K4361115]].
  
 
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<partinfo>BBa_K4361114 parameters</partinfo>
 
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<h3> References </h3>
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[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154

Latest revision as of 15:44, 12 October 2022


Blc operator sequence SDM deletion

This part shows the blc operator sequence after site-directed mutagenesis of the (Part:BBa_K4361111) with primers Part:BBa_K4361112 and Part:BBa_K4361113, resulting in the deletion of the adenine in position 20.

It is known from literature that this mutation disrupts the binding between BlcR and the blc operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.

The mutated blc operator sequence is incorperated in a superfolded GFP production plasmid, see Part:BBa_K4361115.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154