Difference between revisions of "Part:BBa K4180003"
 
(20 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4180003 short</partinfo>
 
<partinfo>BBa_K4180003 short</partinfo>
  
−
SNF1&#916;381-633aa, C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR)
+
<h4>
  
−
??There have two different way to upload??
+
snf1&#916;381-633aa,<!-- C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR)-->
−
[[File:      ??put picture link in here??      ]]
+
−
[[File:      ??put picture link in here??      |200px|thumb|left|    ??you can write the command in this part??    ]]
+
  
 +
C-terminus truncated from amino acid 381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein<br><br>
 +
 +
Citation:  McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200> <br><br>
 +
 +
<span class='h3bb'>Sequence and Features</span>
 +
</h4>
 +
 +
<!--??There have two different way to upload??
 +
[[File:      ??put picture link in here??      ]]
 +
[[File:      ??put picture link in here??      |200px|thumb|left|    ??you can write the command in this part??    ]]-->
 +
[[File:BBa K4180003.png|400px|thumb|left|<h3>;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180003 snf1Δ381-633aa<br>&emsp;&emsp;&emsp;&emsp;&emsp;PCR product on lane 3 compared to<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180002 snf1Δ2-306aa
 +
<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product on lanes 1 and 2 </h3>]]
 +
[[File:BBa K4180003-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa.  PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; comparison </h3>]]
  
  
Line 15: Line 26:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
 
 
<partinfo>BBa_K4180003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4180003 SequenceAndFeatures</partinfo>
  

Latest revision as of 03:22, 12 October 2022


snf1Δ381-633aa C-truncated

snf1Δ381-633aa, C-terminus truncated from amino acid 381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein

Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200>

Sequence and Features

;      BBa K4180003 snf1Δ381-633aa
     PCR product on lane 3 compared to
      BBa K4180002 snf1Δ2-306aa
        PCR product on lanes 1 and 2

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa. PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a          comparison



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]