Difference between revisions of "Part:BBa K4477006:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to get a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene.
+
We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477001 without overburdening the <em>E. coli</em> expressing the gene.
 +
 
 +
We adjusted the RBS sequence to remove restriction sites that would make the RBS incompatible with various assembly methods.
  
 
===Source===
 
===Source===

Latest revision as of 08:35, 12 October 2022


RBS for expression of human/mouse anti-apoB(MDA) antibody light chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene.

We adjusted the RBS sequence to remove restriction sites that would make the RBS incompatible with various assembly methods.

Source

https://salislab.net/software/

References