Difference between revisions of "Part:BBa K4390001"
 
(15 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4390001 short</partinfo>
 
<partinfo>BBa_K4390001 short</partinfo>
  
−
L2U2H09 is a synthetic terminator. The origin is https://www.addgene.org/127042/
+
L2U2H09 is a weak synthetic terminator. It has been shown to function in both ''Escherichia coli'' and ''Bacillus subtilis'', with higher transcription termination efficiency in ''B. subtilis''.
  
  
 +
{|width='80%'
 +
|-
 +
|width=35% valign='top'|
 
[[File:L2U2H09 Structure.png|border|300px|right]]
 
[[File:L2U2H09 Structure.png|border|300px|right]]
 +
| width=50% valign='top' style='border: 1px solid black'|
 +
==L2U2H09 structural information==
 +
 +
This structure of L2U2H09 was generated using mFold DNA folding Form on the UNAFold Web Server. This structure was the likeliest based on loop free-energy predictions, with  &Delta;G = -11.51.
  
 
{| class="wikitable" style="margin:auto"
 
{| class="wikitable" style="margin:auto"
 
|+ Loop Free-Energy Decomposition
 
|+ Loop Free-Energy Decomposition
 
|-
 
|-
−
! Structural element !! &delta;G !! Information
+
! Structural element !! &Delta;G !! Information
 
|-
 
|-
 
| External loop || -0.86 || 15 ss bases & 1 closing helices
 
| External loop || -0.86 || 15 ss bases & 1 closing helices
Line 34: Line 41:
 
|-
 
|-
 
| Hairpin loop || 3.00 || Closing pair is t<sub>12</sub>-a<sub>17</sub>
 
| Hairpin loop || 3.00 || Closing pair is t<sub>12</sub>-a<sub>17</sub>
 +
|}
 
|}
 
|}
  

Latest revision as of 16:42, 5 September 2022


L2U2H09 Terminator

L2U2H09 is a weak synthetic terminator. It has been shown to function in both Escherichia coli and Bacillus subtilis, with higher transcription termination efficiency in B. subtilis.


L2U2H09 Structure.png

L2U2H09 structural information

This structure of L2U2H09 was generated using mFold DNA folding Form on the UNAFold Web Server. This structure was the likeliest based on loop free-energy predictions, with ΔG = -11.51.

Loop Free-Energy Decomposition
Structural element ΔG Information
External loop -0.86 15 ss bases & 1 closing helices
Stack -1.00 External closing pair is a4-t25
Stack -1.44 External closing pair is a5-t24
Stack -2.17 External closing pair is c6-g23
Stack -1.84 External closing pair is g7-c22
Stack -2.24 External closing pair is g8-c21
Stack -1.84 External closing pair is c9-g20
Stack -1.84 External closing pair is c10-g19
Stack -1.28 External closing pair is c11-g18
Helix -13.65 9 base pairs
Hairpin loop 3.00 Closing pair is t12-a17

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]