Difference between revisions of "Part:BBa K4257000:Design"

 
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===Design Notes===
 
===Design Notes===
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To test whether the synthesized polyP can be visualized intracellularly, this part will be over-expressed in E.coli K12 and the engineered strain will be subjected to microscopic examination after staining.
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Highly active version of E. coli native PPK.
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===Source===
 
===Source===
  
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The EcPPK coding sequence was obtained by PCR amplification using the genomic DNA of E.coli K12 as the template.
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The PPK-M coding sequence was obtained by PCR-mediated site-directed mutagenesis using the genomic DNA of E.coli K12 as the template.
  
 
===References===
 
===References===
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Rudat, A. K., et al. (2018). "Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels." 200(6): e00697-00617.

Latest revision as of 16:18, 26 September 2022


PPK-M


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Highly active version of E. coli native PPK.

Source

The PPK-M coding sequence was obtained by PCR-mediated site-directed mutagenesis using the genomic DNA of E.coli K12 as the template.

References

Rudat, A. K., et al. (2018). "Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels." 200(6): e00697-00617.