Difference between revisions of "Part:BBa K238010"

 
 
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<partinfo>BBa_K238010 short</partinfo>
 
<partinfo>BBa_K238010 short</partinfo>
  
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this is an alfa subunit polymerase. it is necessary in E.Coli so that the Vir box can be transcribed properly.
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This RpoA gene from Agrobacterium tumefaciens encodes the alfa subunit of the RNA Polymerase. This is necessary in E. coli so that the Vir box can be transcribed properly, allowing a functional VirA/VirG signaling system.
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RpoA has illegal EcoRI restriction sites in its original sequence. We used site directed mutagenesis to get these sites out. EcoRI was mutated from GAATTC to GAATTT
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 12:45, 8 October 2009

RpoA

This RpoA gene from Agrobacterium tumefaciens encodes the alfa subunit of the RNA Polymerase. This is necessary in E. coli so that the Vir box can be transcribed properly, allowing a functional VirA/VirG signaling system.

RpoA has illegal EcoRI restriction sites in its original sequence. We used site directed mutagenesis to get these sites out. EcoRI was mutated from GAATTC to GAATTT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 607