Difference between revisions of "Part:BBa K4180004"

 
 
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<partinfo>BBa_K4180004 short</partinfo>
 
<partinfo>BBa_K4180004 short</partinfo>
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The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project
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<br>
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eGFP(enhanced green fluorescent protein) is used as a control. The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive<br><br>
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Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212<br>
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<span class='h3bb'>Sequence and Features</span>
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</h4>
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[[File:      ??put picture link in here??      ]]
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[[File:      ??put picture link in here??      |200px|thumb|left|    ??you can write the command in this part??    ]]-->
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[[File:BBa K4180004.jpeg|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180004 eGFP
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<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
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[[File:BBa K4180004-2.jpeg|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do eGFP PCR;  colony#1 (lane2)  has eGFP<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
  
  
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The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K4180004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4180004 SequenceAndFeatures</partinfo>
  

Latest revision as of 03:29, 12 October 2022


enhanced GFP (eGFP)

The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project
eGFP(enhanced green fluorescent protein) is used as a control. The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive

Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212
Sequence and Features

       BBa K4180004 eGFP
         PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do eGFP PCR; colony#1 (lane2) has eGFP
         PCR product



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]