Difference between revisions of "Part:BBa K238000"
 
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ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the reporssor from the promoter region. see reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. ''Genes and development''23: 522-534 (2009)
 
ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the reporssor from the promoter region. see reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. ''Genes and development''23: 522-534 (2009)
  
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there will be an attempt to remove the SpeI site at position 178-183
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the actuall promotor region was later identified with the help of Regine Hengge ang her lab. to see the part for the exact region check out part BBa_K238013.
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making this part was not continued.
  
  

Latest revision as of 11:25, 21 September 2009

YcgF/E regulated promoter region

ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the reporssor from the promoter region. see reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)

the actuall promotor region was later identified with the help of Regine Hengge ang her lab. to see the part for the exact region check out part BBa_K238013.

making this part was not continued.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 178
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]