Difference between revisions of "Part:BBa K4370000:Design"

 
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===Design Notes===
 
===Design Notes===
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The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli. Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimisation for production in E. coli.
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The sequence of the native gene STRBO_RS0123595 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces genomes </i> are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>.
  
  
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===Source===
 
===Source===
  
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This sequence has be synthetized.
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This sequence has been synthetized.
  
 
===References===
 
===References===
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Reference of <i> Streptomyces bottropensis </i> ATCC 25435 genome: GCF_000383595.1_ASM38359v1

Latest revision as of 07:14, 22 August 2022


cbbL_STRBO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 373


Design Notes

The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .


Source

This sequence has been synthetized.

References

Reference of Streptomyces bottropensis ATCC 25435 genome: GCF_000383595.1_ASM38359v1