Difference between revisions of "Part:BBa K4291016"

 
 
(4 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
 
J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro-amilGFP- rrnB T1 ter- T7Te ter
 
J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro-amilGFP- rrnB T1 ter- T7Te ter
 +
 +
== Profile ==
 +
Name: CuR-CopA-amilGFP
 +
 +
Base Pairs: 1703 bp
 +
 +
Origin: E.coli, genome
 +
 +
Properties: Monitor the Cu concentration
 +
 +
== Usage and biology ==
 +
The plasmid was designed to monitor the cooper concentration using the amilGFP intensity. The CueR which belongs to mercury resistance regulator (MerR) family response the mental ions including cooper and silver. Meanwhile, the CueR control the expression of CopA promoter. In the MerR family, there are other metal-responsive metal-regulators including ZntR, CadR, CoaR which response to the heavy metal zinc, cadmium and cobalt, respectively. All of the metal-regulators has the same property that these regulators could balance the heavy metal ions in bacteria via regulating the transcription of heavy-metal-resistance genes.
 +
[[File:T--Canton HS--BBa K4291016-figure 11.jpg|500px|thumb|center|Figure 1. map of pUC19-CuR-CopA-amilGFP]]
 +
 +
The components of this plasmid are described as follows:
 +
==BBa_K4291012 ==
 +
Name: CueR
 +
 +
Base Pairs: 408 bp
 +
 +
Origin: E.coli, genome
 +
 +
Properties: regulate the cooper efflux
 +
 +
== Usage and biology ==
 +
BBa_K4291012 is an encoding sequence of cooper efflux regulator (CuR) which is sensitive to metal ions such as Cu+, Ag+, Au+. In addition, CuR controls the transcription of copA in response the cooper salt.
 +
 +
== BBa_K4291013 ==
 +
Name: CopA promoter
 +
 +
Base Pairs: 287 bp
 +
 +
Origin: E.coli, genome
 +
 +
Properties: Cu+-transporting ATPase pump
 +
 +
== Usage and biology ==
 +
BBa_K4291013 is an encoding sequence copA which is a cooper-transporter P-type ATPase pump. The expression of copA is improved when exposed to metal ions such as silver and cupric ions due to eliminating the CuR effect.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:36, 30 September 2022


CueR-CopA-amilGFP

J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro-amilGFP- rrnB T1 ter- T7Te ter

Profile

Name: CuR-CopA-amilGFP

Base Pairs: 1703 bp

Origin: E.coli, genome

Properties: Monitor the Cu concentration

Usage and biology

The plasmid was designed to monitor the cooper concentration using the amilGFP intensity. The CueR which belongs to mercury resistance regulator (MerR) family response the mental ions including cooper and silver. Meanwhile, the CueR control the expression of CopA promoter. In the MerR family, there are other metal-responsive metal-regulators including ZntR, CadR, CoaR which response to the heavy metal zinc, cadmium and cobalt, respectively. All of the metal-regulators has the same property that these regulators could balance the heavy metal ions in bacteria via regulating the transcription of heavy-metal-resistance genes.

Figure 1. map of pUC19-CuR-CopA-amilGFP

The components of this plasmid are described as follows:

BBa_K4291012

Name: CueR

Base Pairs: 408 bp

Origin: E.coli, genome

Properties: regulate the cooper efflux

Usage and biology

BBa_K4291012 is an encoding sequence of cooper efflux regulator (CuR) which is sensitive to metal ions such as Cu+, Ag+, Au+. In addition, CuR controls the transcription of copA in response the cooper salt.

BBa_K4291013

Name: CopA promoter

Base Pairs: 287 bp

Origin: E.coli, genome

Properties: Cu+-transporting ATPase pump

Usage and biology

BBa_K4291013 is an encoding sequence copA which is a cooper-transporter P-type ATPase pump. The expression of copA is improved when exposed to metal ions such as silver and cupric ions due to eliminating the CuR effect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]