Difference between revisions of "Part:BBa K4268001:Experience"

 
 
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The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha; cells.  After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
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how you used this part and how it worked out.
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[[File: T-suny-oneonta-t7-capsid assembly protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.]]
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The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
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===Applications of BBa_K4268001===
 
===Applications of BBa_K4268001===
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Latest revision as of 18:21, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.

The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.


Applications of BBa_K4268001

User Reviews

UNIQcb34491ffeabdbda-partinfo-00000000-QINU UNIQcb34491ffeabdbda-partinfo-00000001-QINU