Difference between revisions of "Part:BBa K4161002"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4161002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4161002 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K4161002 parameters</partinfo> | <partinfo>BBa_K4161002 parameters</partinfo> | ||
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| + | ===Source=== | ||
| + | The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK) | ||
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| + | ===Reference=== | ||
| + | Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231. | ||
Latest revision as of 12:10, 20 July 2022
JMK-H2
JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)
Reference
Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.