Difference between revisions of "Part:BBa K4161001"
 
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<partinfo>BBa_K4161001 short</partinfo>
 
<partinfo>BBa_K4161001 short</partinfo>
  
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20IpaD is a VHH antibody wich targets the IpaD antigen, which makes up the needle tip of Type III secretion system of Shigella. The DNA sequence is reversed from the 20IpaD protein sequence and codon optimized for expression in E. coli.
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20IpaD is a VHH antibody wich targets the IpaD antigen that makes up the needle tip of Type III secretion system of Shigella. The DNA sequence is reversed from the 20IpaD protein sequence and codon optimized for expression in E. coli.
  
 
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<partinfo>BBa_K4161001 parameters</partinfo>
 
<partinfo>BBa_K4161001 parameters</partinfo>
 
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===Source===
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The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)
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===Reference===
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Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.

Latest revision as of 12:06, 20 July 2022


20IpaD

20IpaD is a VHH antibody wich targets the IpaD antigen that makes up the needle tip of Type III secretion system of Shigella. The DNA sequence is reversed from the 20IpaD protein sequence and codon optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)

Reference

Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.