Difference between revisions of "Part:BBa K4421027"
 
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Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ  2)transmembrane domain: CD8TM  
 
Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ  2)transmembrane domain: CD8TM  
 
3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all  
 
3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all  
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kinds of scFvs which can be genetated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in <partinfo>BBa_K4421003</partinfo>.
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kinds of scFvs which can be generated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in <partinfo>BBa_K4421003</partinfo>.
  
 
<div><ul>  
 
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   <li style="display: inline-block;">  [[File:CAR library.jpeg|thumb|none|250px|<b></b> The structure of CAR_library]]  
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   <li style="display: inline-block;">  [[File:CAR-library1.jpeg|thumb|none|600px|<b></b> The structure of CAR_library]]  
 
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Latest revision as of 01:33, 7 October 2022


CAR_library

As we have mentioned in BBa_K4421003, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.

Structure of CAR_library

Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all kinds of scFvs which can be generated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in BBa_K4421003.

  • The structure of CAR_library


Theoretical base

According to the clonal selection theory, a healthy adult with a normal immune system could produce a very large number of antibodies against all antigens beforehand, including heterogeneous tumor antigens. So we can utilize these antibodies to form an antibody or scFv library which aims for almost all antigens.

Special Note

Since our part BBa_K4421003 is a general concept without a specific sequence, we use cetuximab scfv (BBa_K4421011) to serve as a placeholder. In other words, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 1832
    Illegal NgoMIV site found at 1898
    Illegal NgoMIV site found at 2065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1439
    Illegal SapI.rc site found at 1996