Difference between revisions of "Part:BBa K4159004"
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6xHis-tag that can be used before the gene of interest. The tag was added between the RBS and the DAPRin sequence. | 6xHis-tag that can be used before the gene of interest. The tag was added between the RBS and the DAPRin sequence. | ||
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| + | Characterization: DARPins can be easily purified with the Ni-NTA purification kit when they have this His-tag connected to them. We have done ribosome display as the binding assay for our DARPins, including this His-tag, as well as purified DARPins with the His-tag | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K4159004 parameters</partinfo> | <partinfo>BBa_K4159004 parameters</partinfo> | ||
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Latest revision as of 16:52, 1 October 2022
6xHis-tag
6xHis-tag that can be used before the gene of interest. The tag was added between the RBS and the DAPRin sequence.
Characterization: DARPins can be easily purified with the Ni-NTA purification kit when they have this His-tag connected to them. We have done ribosome display as the binding assay for our DARPins, including this His-tag, as well as purified DARPins with the His-tag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]