Difference between revisions of "Part:BBa K4159002"

 
 
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===Usage and Biology===
 
===Usage and Biology===
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Description: This is a common consensus promoter that can be induced by IPTG. The following sequence after the promoter sequence is derived from the plasmid sequence used in the Chen et al. (2021) paper.
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The part has been used for ribosome display as well as the promoter for GFP binding DARPin expression. Check out the parts BBa_K4159012 and BBa_K4159000 for a more detailed description.
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4159002 parameters</partinfo>
 
<partinfo>BBa_K4159002 parameters</partinfo>
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Latest revision as of 16:25, 1 October 2022


T7 promoter

Promoter for bacteriophage T7 RNA polymerase. With added stem-loop containing XbaI cutting site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]