Difference between revisions of "Part:BBa K4429004:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
-
 
  
 +
Ensuring the correct V->C region interface. Used Uniprot IMGT entry to find where constant region starts.
 +
 +
Removing BioBrick illegal restriction sites using silent mutation in SnapGene.
  
  
 
===Source===
 
===Source===
  
−
-
+
Variable region protein sequence from PDB 7CTH [1], reverse translated using E. coli codon usage table in SnapGene, constant region from IMGT [2]. The entire light chain was then codon optimised for E. coli K12 using IDT codon optimisation tool.
  
 
===References===
 
===References===
 +
 +
[1] https://www.rcsb.org/structure/7CTH
 +
 +
[2] https://www.imgt.org/ligmdb/view?id=X51755

Latest revision as of 03:15, 14 October 2022


C10 IgG1L LC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensuring the correct V->C region interface. Used Uniprot IMGT entry to find where constant region starts.

Removing BioBrick illegal restriction sites using silent mutation in SnapGene.


Source

Variable region protein sequence from PDB 7CTH [1], reverse translated using E. coli codon usage table in SnapGene, constant region from IMGT [2]. The entire light chain was then codon optimised for E. coli K12 using IDT codon optimisation tool.

References

[1] https://www.rcsb.org/structure/7CTH

[2] https://www.imgt.org/ligmdb/view?id=X51755