Difference between revisions of "Part:BBa K176026:Design"
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Design: | Design: | ||
− | pCon 0.70(J23101) | + | pCon 0.70(J23101) generates LuxR |
− | pLux/Tet(K176000) | + | pLux/Tet(K176000) generates GFP, activated by LuxR and AHL, repressed by TetR and no aTc |
===Source=== | ===Source=== |
Latest revision as of 06:32, 30 July 2009
TetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Illegal BsaI.rc site found at 1729
Design Notes
Input: AHL
Output: GFP
Design:
pCon 0.70(J23101) generates LuxR
pLux/Tet(K176000) generates GFP, activated by LuxR and AHL, repressed by TetR and no aTc
Source
K176025 K176002