Difference between revisions of "Part:BBa K194001"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K194001 short</partinfo>
 
<partinfo>BBa_K194001 short</partinfo>
  
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Green Fluorescent Protein (GFP) codonoptimized for yeast.
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This constitutively expressed GFP, originally from Aequorea victoria was codon optimized and developed as a reporter for gene expression in Saccharomyces cerevisiae according to Cormak et al (1997). In addition, two amino acid changes were included enabling a far more bright florescence compared to the wild type in E.coli. Maximum absorbance: 490 nm
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<p align="justify">By expanding the host organism, this is an improvement of an existing biobrick:
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<html>
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<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040" target="_blank">BBa_E0040</a>
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</html>
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</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:45, 22 October 2009

GFP (a yeast- and FACS-optimized GFP variant),

This constitutively expressed GFP, originally from Aequorea victoria was codon optimized and developed as a reporter for gene expression in Saccharomyces cerevisiae according to Cormak et al (1997). In addition, two amino acid changes were included enabling a far more bright florescence compared to the wild type in E.coli. Maximum absorbance: 490 nm

By expanding the host organism, this is an improvement of an existing biobrick: BBa_E0040

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644