Difference between revisions of "Part:BBa K1682012:Experience"

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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K1682012===
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===Lambert_GA 2021===
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==EXPERIENCE (Lambert_GA 2021)==
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Utilizing the phosphate range of 0-100μM and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the [https://parts.igem.org/Part:BBa_K3725110 BBa_K3725110] phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.  
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Utilizing the modified phosphate range and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.  
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[[File:ASDJFCJBASJHBFD.png|thumb|center|800px|Figure 2. Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 100μM.  ]]
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[[File:ASDJFCJBASJHBFD.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 150μM.  ]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 20:37, 17 December 2021


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Please enter how you used this part and how it worked out.

Lambert_GA 2021

Utilizing the phosphate range of 0-100μM and characterization protocol (See: Modified Protocol), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.

Figure 1. Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 150μM.

User Reviews

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