Difference between revisions of "Part:BBa K116401:Experience"

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===Lambert_GA 2021===
 
===Lambert_GA 2021===
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Utilizing the modified phosphate range and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.  
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Utilizing the phosphate range of 0-100μM and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the [https://parts.igem.org/Part:BBa_K2447000 BBa_K2447000] phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.  
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[[File:Fjasknfkncjak.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM. ]]
 
[[File:Fjasknfkncjak.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM. ]]

Latest revision as of 20:36, 17 December 2021


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Lambert_GA 2021

Utilizing the phosphate range of 0-100μM and characterization protocol (See: Modified Protocol), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.


Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM.

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