Difference between revisions of "Part:BBa K4060001:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
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The fragment is ligated by 2021_NYCU_Taipei using the iGEM kit.
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a
+
  
 
===References===
 
===References===

Latest revision as of 13:10, 7 December 2021


MazF gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal SpeI site found at 363
    Illegal NotI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence confirmed.

Source

The fragment is ligated by 2021_NYCU_Taipei using the iGEM kit.

References