Difference between revisions of "Part:BBa K4060021"
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Latest revision as of 01:34, 12 October 2022


pelB leader sequence with aprN (without native signal peptide sequence)

aprN (without natural singal peptide) + pelB. pelB leader sequence which is a gene fragment of pelB can help to direct to E. coli periplasmic membrane. The aprN gene is the gene of Nattokinase. Nattokinase (subtilisin NAT) is a kind of extracellular alkaline serine protease, which has the ability to prevent deep vein thrombosis (DVT) due to its fibrinolytic activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 67
    Illegal NotI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 67
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 349