Difference between revisions of "Part:BBa K4060020:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | We use PCR technique to clone the gene fragment from genomic DNA of Bacillus subtilis natto. We added restriction enzyme cutting site, and ligated into pET-21a(+) to establish cloning vector. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | genomic DNA of Bacillus subtilis | + | The source of the gene is from genomic DNA of Bacillus subtilis natto. |
| + | Bacillus subtilis natto HS01 (a generous gift from Dr. Yi-Huang Hsueh) | ||
===References=== | ===References=== | ||
Latest revision as of 08:12, 4 December 2021
aprN (Nattokinase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 223
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 357
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 361
Design Notes
We use PCR technique to clone the gene fragment from genomic DNA of Bacillus subtilis natto. We added restriction enzyme cutting site, and ligated into pET-21a(+) to establish cloning vector.
Source
The source of the gene is from genomic DNA of Bacillus subtilis natto. Bacillus subtilis natto HS01 (a generous gift from Dr. Yi-Huang Hsueh)