Difference between revisions of "Part:BBa K4060105"
 
Line 3: Line 3:
 
<partinfo>BBa_K4060105 short</partinfo>
 
<partinfo>BBa_K4060105 short</partinfo>
  
−
BBa_K4060101 with thermometer RBS
+
<partinfo>BBa_K4060101</partinfo> with thermometer RBS
  
−
This construct is our intermediate. For more information, please visit our results page
+
The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS <partinfo>BBa_K115002</partinfo>. We digested the vector fragment <partinfo>BBa_K115002</partinfo> with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
  
  

Latest revision as of 14:28, 7 December 2021


-- No description --

BBa_K4060101 with thermometer RBS

The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]